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ELISA-Entwicklung GmbH & Co. KG
The laboratory for professional assay development

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Protein Purification

Protein purification from biological fluids, cell culture media or tissue extracts can be performed on its own or in combination with the development of relevant test procedures.
 
Specific proteins or peptides are essential for the preparation of:
  • antigen for antibody production
  • assay standards in test systems
  • tracer (labelled antigen) in assays.
The following purification methods are established in our laboratory:
  • specific extraction procedures
  • column chromatographic procedures (FPLC) including:
    gel filtration, anion and cation exchange, affinity chromatography, reverse-phase chromatography
  • electrophoresis
  • dialysis.
   
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          «Peptide assays»
          Assay validation
          Sample measurement
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          Protein purification
          Antibody production
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